Ability of insulin to increase calcium uptake by adipocyte endoplasmic reticulum.
نویسندگان
چکیده
منابع مشابه
Ability of insulin to increase calcium binding by adipocyte plasma membranes.
Calcium specifically binds to adipocyte plasma membranes, demonstrating two classes of binding sites having affinity constants of 4.5 x 10(4) M-1 and 2.0 x 10(3) M-1. Insulin (100 microunits/ml) added directly to the isolated plasma membranes caused no alteration in calcium binding, whereas incubation of the adipocytes with 100 microunits/ml of insulin resulted in a 2.50 +/- 1.6% increase in ca...
متن کاملThe coupling of plasma membrane calcium entry to calcium uptake by endoplasmic reticulum and mitochondria.
Cross-talk between organelles and plasma membrane Ca(2+) channels is essential for modulation of the cytosolic Ca(2+) ([Ca(2+)]C) signals, but such modulation may differ among cells. In chromaffin cells Ca(2+) entry through voltage-operated channels induces calcium release from the endoplasmic reticulum (ER) that amplifies the signal. [Ca(2+)]C microdomains as high as 20-50 μm are sensed by sub...
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Objective(s): To explore whether endoplasmic reticulum (ER) stress regulates inflammation in adipose tissue of obese rats via TLR4 signaling. Materials and Methods: Sprague Dawley rats were randomly divided into four groups, and body weight, food intake, and free fatty acids (FFA) were measured. Real-time PCR and Western blot were used to determine mRNA or protein expression of TLR4, TRAF6, IKK...
متن کاملThe regulation of endoplasmic reticulum calcium uptake of adipocytes by cytoplasmic calcium.
The endoplasmic reticulum of adipocytes can accumulate calcium by an active transport system linked to (Ca2+ + Mg2+)-ATPase activity (Black, B. L., Jarett, L., and McDonald, J. M. (1980) Biochim. Biophys. Acta 596, 359-371). The ability of this transport system to regulate intracellular calcium levels was tested by incubating adipocytes with various concentrations of calcium and measuring the c...
متن کاملEndoplasmic Reticulum Stress Regulates Adipocyte Resistin Expression
OBJECTIVE Resistin is a secreted polypeptide that impairs glucose metabolism and, in rodents, is derived exclusively from adipocytes. In murine obesity, resistin circulates at elevated levels but its gene expression in adipose tissue is paradoxically reduced. The mechanism behind the downregulation of resistin mRNA is poorly understood. We investigated whether endoplasmic reticulum (ER) stress,...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34829-9